Determination of some Immunological and Hematological Parameters in Patients with Rheumatoid Arthritis in Khartoum Teaching Hospital, 2011

Abstract

This is a descriptive cross sectional study which was carried out to determine some immunological parameters (anti-cyclic citrullinated peptide anti-ccp and rheumatoid factor RF ) and some hematological parameters ( complete blood count and erythrocyte sedimentation rate ) in patients with rheumatoid arthritis in Khartoum teaching hospital in the period from January to May 2011. For this study the total of ninety four rheumatoid arthritis patient samples ( n=94 ) have been used in this study , and ninety four samples (n=94 ) from normal control individuals were tested for anti-cyclic citrullinated peptides by ELISA, rheumatoid factor , complete blood count by Sysmex and erythrocyte sedimentation rate which can be divided as follows : fifty samples were tested for anti-ccp alongside with their fifty normal control individuals and forty four RA samples were tested for ESR ,CBC and RF along with their normal control individuals. The results showed that all the patients with rheumatoid arthritis ( 100% ) were positive for anti-cyclic citrullinated peptides compared to normal control individuals, And forty two rheumatoid arthritis patients were positive for rheumatoid factor latex test and two were negative.Also in the present study the results of forty four patients of rheumatoid arthritis show a slight decrease in the hemoglobin concentration mean Hb (12.1 g/dl ±1.5 SD), red blood cells count mean RBCs (4.5×10^12/L±0.4SD) and packed cell volume mean PCV (37.9% ±4.0 SD) compared to normal control individuals and there were ten patients had their hemoglobin below the normal range or anemic mean Hb ( 10.1g/dl),and some with lymphopenia mean lymphocyte count (22.7%) as one of the extra-articular complication of rheumatoid arthritis and the erythrocyte sedimentation rate was markedly (P.value=0.00) elevated mean ESR (57.4 mm/ 1hr ± 34.8 SD) in RA. Also female (76.59 %) were found to be predominantly more affected than male (23.15%) .