Physicochemical Characterization of FlavonoidS from (Pulicaria Crispa)

Abstract

In this study four flavonoid compounds were isolated. Their structures were elucidated, and their biological activity was evaluated. Dry powdered aerial parts of Pulicaria crispa were extracted with aqueous methanol (70%). The crude extract was fractionated by polyamide and sephadex columns and further purification was accomplished via paper chromatography. Aerial parts of Pulicaria crispa were subjected to hytochemical screening and the qualitative tests were positive to flavonoids, steroids, alkaloids, terpenes, tannins and glycosides. Four flavonoid compounds were reported for the first time from the Sudanese material of Pulicaria crispa, and their structures were elucidated by spectroscopy techniques (UV, FTIR, 1HNMR and MS). Compound I was isolated as colorless powder: OH O HO OH O- Rh. OH O )I( Compound II was isolated as a pale yellow solid powder: CH3 OH O HO OH OH O )II( xv OCH3 Compound III was isolated as a white solid powder: OCH3 O OCH3 OH OH O )III( Compound IV was isolated as yellow solid powder: OH O HO OH OH OH O )IV( The crude methanolic extract and fractions (obtained from polyamide column) were evaluated for their antioxidant activity and excellent results were obtained.