An in-vitro Study On the Antibacterial Effect of Garlic (Allium sativum) Extract Against Staphylococcus aureus, Pseudomonas aeruginosa and Proteus species

Abstract

Staphelococcus aureus. Pseudomonas and Proteus species are most common cause of hospital acquired or nosocomial infection, especially in patients with post-surgical wound, diabetic and immunocompromised. This study was conducted in the Royal Medical Laboratory in Wad Medani during July to September 2013. It was aimed to investigate the antibacterial activity of garlic extract against three bacterial species (S. aureus. Pseudomonas aeroginosa and Proteus species). Thirty five wound swab samples were collected from patients with post-surgical and diabetic wounds who were hospitalized in Wad- Medani Teaching Hospital. The 35 samples were collected and cultured in the laboratory, 8 S. aureus, 7 Pseudomonas and 5 were Proteus species were isolated from the collected samples . Sensitivity tests were done to the 20 isolates by using two concentrations of fresh garlic extract (1 g/ml, 500 mg/ml), on Muller Hinton agar medium. The results showed that; at the concentration of 1 g/ml, the mean diameter of zone was 30.8 for S. aureus, 28.5 for Pseudomonas species, 23 for Proteus species and 0 for pseudomonas aeroginosa. At the concentration 500 mg/ ml the mean diameter of zone was 24.8, 24.5, 19 and 0 respectively. Overall results of this study indicate that Allium sativum have an activity that can prevent the growth of S. aureus, Pseudomonas and Proteus species on Muller concentrations. Hinton agar (in-vitro) at different