Detection of Extended–spectrum Beta Lactamase Genes in Urinary Tract Isolated Bacteria among Pregnant Women

Abstract

The aim of this study was to detect extended spectrum β-lactamase (ESBL) in bacterial isolated from urine collected from urinary tract infected pregnant women. A total of 100 midstream urine samples were collected from Omdurman Militory Hospital during April 2013. Specimens were cultured on CLED agar to identify UTI causative bacteria by colonial morphology, Gram stain and routine biochemical tests. Out of 100 urine specimen; 56(56%) specimen gave significant bacterial growth, the Grame negative bacilli were 42(75%) and 14(25%) Gram positive. The most frequenty Gram negative was K. oxytoca 15 (26.8%) and E. coli 10(17.9%), while the most abundant Gram positive was S. saprophyticus. The susceptibility testing, 5 isolates were resistant to 3rd generation cephalosporines (ceftazidime, ceftriaone and cefotaxime) as follow: 2 K. pneumoniae ,2 E. coli and 1 P. vulgaris. ESBL production test done for all bacterial resistance to 3rd generation cephalosporin and then confirmed by combination test by using Double disc synergy test 5(11.9% ) isolates gave positive result. The PCR was done for 5 isolates using TEM, SHV, and CTX-M primers, TEM gene was the most predominant one 5(100%) followed by CTX-M 3(60% )and the least one was SHV gene 2(40% ).