Detection of Cytomegalovirus in Product of Conception Using Histopathological, Immunohistochemical ,and Serological methods

Abstract

This is a descriptive prospective hospital base study, was conducted in Damazine Military hospital - Blue Nile state, during the period from March to July 2012. Aimed to detect CMV in product of conception (aborted tissue) using histochemical,serology and immunohistochemical methods. Fifty samples were collected from females suffering of miscarriage for one time or recurrent attending Damazine military hospital, their ages were ranged between16 to 41 year, with mean age 28.5 years. 5o sample (biopsy and serum) ,biopsies were fixed in formalin,prossed in paraffin and cut using routary microtom, two section were prepared from each block. One section was stained using Avidin-Biotin technique for immunohistochemical method to detect CMV antibodies, another section was stained using Heamatoxylene and Eosin for histological method to detect CMV inclusion bodies, serum samples were used for CMV (IgG) using ELISA commercial diagnostic kits (DRG Instruments GmbH. Germany), data were analyzed using SPSS computer program. Immunohistochemestry result releave 10(20%) sections were positive, while 40(80%) were negative. Histopathological results showed 23(46%).While 27(54%) no changes. IV changes of CMV in CMV IgG antibodies were present in the serum samples of all women tested with ELISA. This study showed insignificant relation between age, ethnic origin, economic status, education level, occupation, number of parity, blood transfusion, Intra Uterine Fetal Death (IUFD). Congenital malformation and Immunohistochemical result. The study concluded that The CMV IgG results indicate high frequency of past HCMV infection in all tested groups; Histopathology still represents accurate results of CMV infection.