Molecular Detection of Helicobacter pylori in Patients with Gastroduodenitis and Peptic Ulcer in Khartoum State

Abstract

The aim of this study was to detect H. pylori 16S rRNA gene by using polymerase chain reaction (PCR), in patients with gastroduodenitis and peptic ulcer attending the outpatients clinics of Omdurma Military Teaching Hospital and Omdurman Teaching Hospital during the period from April to August, 2013. A total of 57 patients (n=57) of both genders and ages ranging from 21 to 80 years were included in this descriptive , cross sectional study . Endoscopy biopsy samples were collected, and examined for H. pylori DNA by PCR technique . Out of the 57 patients examined , 12 were found positive (21.1%) for H. pylori DNA. This findings indicated that H. pylori could possibly associated with gastroduodenitis and peptic ulcer in Khartoum area .