Bacterial Nosocomial Infection in Patients with Different Tumors Hospitalized in Radiation & Isotopes Centre Khartoum

Abstract

This is study was carried out to assess the level of bacterial infection acquired by cancer patients during hospitalization, identifying their causes and to evaluate the antibiotic used for its treatment. Also was carried to evaluate the efficiency of disinfectants and detergents used for hospital environmental sanitation. One hundred sixty five clinical samples were collected from patients suffering of different infection bacteriological laboratory investigation. They included 118 (71.5%) urine samples, 14 (8.4%) surgical wound swabs, 9 (5.4%) Ear swabs, 10 (6.0%) Throat swabs and 14 (8.4%) samples from other sites of infection. Floor swabs and air samples were also taken from patient’s wards.. The identification of the isolated organisms utilized cultural characteristics, Gram's reaction, and biochemical tests. A total of hundred organisms were isolated and identified. 9 (9%) Staphylococci aureus, 30 (30%) Coagulase-negative Staphylocoooi, 25 (25%) E. colin, 7 (7%) Klebsiella pneumoiae, 7 (7%) Proteus mirabilis, 6 (6%) Peudomonas aeruginosa, 2 (2%) Enterobacter spp., 7 (7%) Candida albican. 3 (3%) Enterococci spp., 3 (3%) Streptococcus pyogenes, 1 (1%) Salmonella paratyphi B. Antimicrobial susceptibility testing was carried out by disk diffusion method on Mueller Hinton agar medium by Kirby-Bauer technique. Gentamicin and streptomycin were found to be the most effective antimicrobial agent. All E.coli strains was found to be 100% susceptibility to gentamicin and streptomycin. Most coagulase-negative Staphylocoooi strains showed 100% and 70-90 % susceptibility gentamicin, streptomycin and ampicllin, coloxacillin and penicillin. Settle plate technique was used to obtained samples before and after surgical theatre disinfection by formalin and the bioload was found to be 1600/m3 and 400/m3, respectively.