Frequancy of Gardnerella vaginalis among Women of Reproductive Age in Riyadh, Kingdom of Saudi Arabia

Abstract

The aim of this study was to detect Gardnerella vaginalis, the main causative agent of bacterial vaginosis, more than other opportunistic anaerobic organisms that colonized the vagina. Out of 150 women who visited the Obstetrics and Gynecology Clinic in Rabia Hospital, Riyadh- Saudi Arabia during the period between July 2011 to June 2012, were included in this study. 92/150 (61.3%) have vaginal infection. Gardnerella vaginalis was n=74/92 (80.4%) was isolated from women with bacterial vaginosis, while Candida species, Streptococcus species and Trichomomas vaginalis were 16.3%, 2.2% and 1.1 % respectively. Isolated Gardnerella vaginalis were examined for biotype (hippurate hydrolysis, catalase and oxidase activity) and genotype with molecular technique (PCR) Amplified ribosomal DNA (16S rDNA). This work concluded that Gardnrella vaginalis is most bacterial vaginosis causative agent in women of reproductive ages in Riyadh –Saudi Arabia.