EFFICIENCY OF DWARF BEE HONEY (Apis florea) AGAINST THREE SPECIES OF WOUND INFECTING BACTERIA

Abstract

The study was carried out under control laboratory conditions at Faculty of Veterinary Medicine of Khartoum University during January 2005, to evaluate the effect of the Dwarf bee honey( Apis florea) and chemical antibiotic Gentamicine on the three species of wounds bacteria relates to Family: Enterobacteria. Tow samples of honey were collected from wild nests from Khartoum North area.(Elfakee Hashim and Elgiley sites). Chemical analysis of the different components of the samples was carried out immediately at Industrial Research and Consultancy Center (IRCC).The result confirmed the previous studies. The Bacteria species brought from Sudan National Health Laboratory. Honey concentrations were prepared by diluted solution of honey in distilled water, and three concentrations of honey were prepared (25%, 50% and 100%). Disk of filter paper equal in size to antibiotic disks (Gentamicin10mg) were embedded in different concentrations of honey for 24hours in 37°C. Disk sensitivity test was chosen to conducted the study. Experimental units were arranged in Completely Randomized Black Design (CRBD) with three replicates. Honey at 100% concentration after 24h incubated at 37°C gave the highest mortality almost similar to chemical antibiotic (Gentamicine10mg) on Escherichia coli and Proteus mirabilis but in Klebsiella pneumonia the effect was equivalent to chemical antibiotic.