Detection of Herpes Simplex Virus 1 and 2 among Renal Transplant Recipients in Khartoum

Abstract

This study was carried out in Khartoum State from June to December 2013. The aim of this study was to detect herpes simplex virus 1and 2 in Renal Transplant Recipients. A total of 50 patients were included in this study. The patients ages ranges from 18-65 years, and from both gender. From the study participants, plasma samples were collected, and tested for HSV1and2 IgM antibody using Enzyme Linked Immune Sorbent Assay (ELISA) and for HSV DNA by Polymerase Chain Reaction (PCR). Out of the 50 patients, 1 (2%) was found to be positive by ELISA, while, 11(22%) were positive by PCR. Out of 11 positive patient, 5 (45%) infected by HSV-1 and 6 (55%) infected by HSV-2. From the above finding we concluded that, there were a high percentage of herpes simplex viruses in renal transplant recipient in Sudan. PCR give more reliable results in detection of HSV. We recommended the screening of renal patients for HSV, and use of HSV DNA as a diagnostic tool.