Cervical Cellular Changes Associated with Microbial Infections

Abstract

This study was carried in Khartoum state during the period from April 2009 to march 2010, to investigate the association between the cervical cellular changes and microbial infections. One hundred and ten high vaginal swabs and Papanicolaou smears were collected from patients attended Um El moamineen charity gynecologic clinic. The swabs were cultured on blood agar, chocolate blood agar and MaCconkey agar for primary isolation of the pathogens. Identification of isolates was done by colonial morphology, Gram's staining technique and biochemical tests. The cervical smears firstly fixed in 95% ethyl alcohol and then stained with Pap stain and screened for cell changes or atypia if present. The study reveled that Bacterial infection were the main cause of vaginintis followed by Candida species infections, other bacteria were found also to be source of infection but in varies percentages like Staphalococuus Auerus, streptococcus species, E.coli, N. gonarrhea and other types were also isolated. After cytology screening 55.5% showed normal smear, 40% showed inflammatory cells and infection with different types (bacterial, fungal, parasitic and some showed non specific inflammatory changes), 3.6% showed dyskaryosis and only 0.9% showed invasive squamous cell carcinoma. The statistic results showed a significant relationship between microbial infections and cervical cellular changes.