Detection of air pollution and temperature stress on cotton plant utilising laser induced fluorescence technique [LIF]

Abstract

In this thesis, laser induced fluorescence (LIF) technique, was tested for detecting changes in cotton plant due to environmental factors: temperature, time during the day hours and pollution stress. Fluorescence measurements were carried out to determine the fluorescence intensity ratio (FIR) F685/F730 with respect to change in temperature and during the day hours. It has been noticed that this ratio(FIR) decreases when temperature increases. Also the ratio (FIR) decreases when pollution concentration increases. Laser induced fluorescence can be a suitable method of monitoring changes in chlorophyll content in a non-destructive way in cotton plant during physiological changes during the day hours. Results of this study demonstrate the capability of early detection of cotton influences due to air pollution and environmental stresses, using laser induced fluorescence technique.