Investigation of the Flavonoid of Some Medicinal Plants

Abstract

In this study phenolic compounds were extracted from Acacia nilotica var nilotica , Acacia nilotica var astringens (Known locally as sunat) and Hyphaere thebaica (Known as dome) using ethanol. The phenolic compounds were separated by thin layer chromatography (TLC). Compound 1 was isolated from Acacia nilotica var nilotica using; butanol: Acetic acid: water (5:4:1). The chemical structure of compound 1 was deduced on the basis of its infrared, ultraviolet, nuclear magnetic resonance and mass spectra. Compound 1 was found to be a flavanone with the following structure: HO HO O rh.glu. rh.glu O The study of the effect of the crude product of Acacia nilotica var nilotica and compound 1 on (G+,G-) bacteria revealed that compound 1 was more potent than the crude extract against Staphylococcus aureus, Escherichia coli, and Proteus vulgaris. Compound 2 was isolated from Acacia nilotica astringens using benzene: Methanol (4:1). The chemical structure of compound 2 was elucidated on the basis of its spectral data. Compound 2 was found to be a flavanone and it was assigned the following tentative structure, O HO HO CH 3 O OCH 3 CH 3 The study of the effect of the crude product of Acacia nilotica stringens and compound 2 (G+,G-) bacteria revealed that compound 2 was more effective than the crude extract against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Proteus vulgaris and Pseudomonas aeruginosa. Compound 3 was isolated from Hyphaene thebaica by using; benzene: Methanol (2:1). The structure of compound 3 was deduced on the basis of its spectral data. Compound 3 was found be to a flavone and the following structure was suggested: OH OCH5 2 OCH5 2 O OCH5 2 OCH5O 2 OH OCH5 2 2 OCH5 OCH5 2 viii OCH5 2 The study of the effect of the crude product Hyphaene thebaica and compound 3 on (G+,G-) bacteria revealed that compound 3 was more effective than the crude product against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa.