Detection of Salmonella paratyphi B in blood Specimens by Real- time S Polymerase Chain Reaction

Abstract

This study was carriedout in the period from May 2007- March 2008 to detect the presence of Salmonella paratyphi B in patient’s suspected to have paratyphoid fever. Fourty seven blood specimens were collected from patients attended Umdorman Teaching Hospital, Khartoum teaching hospital, Alribat and Jafar Ibn Ouf Pediatric's hospitals. Bacterial DNA was extracted from each blood specimen using Phenol –Chloroform technique. Quantica thermocycler was used to detect Salmonella paratyphi B. The result revealed that 22 (46.8%) specimens were positive and 25 (53.2%) were negative. The study concluded that the real-time PCR technique facilitates detection of bacterial pathogens without bacteriological culture.