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Please use this identifier to cite or link to this item: https://repository.sustech.edu/handle/123456789/3385
Title: Detection of Salmonella paratyphi C in Blood Specimens by Real Time Polymerase Chain Reaction Technique
Authors: Ali, Ghada Ahmed Mohammed
Supervisor,- Humodi Ahmed Saeed
Keywords: Salmonella paratyphi C
Blood-Specimens
Issue Date: 1-Jan-2008
Publisher: Sudan University of Science and Technology
Citation: Ali,Ghada Ahmed Mohammed.Detection of Salmonella paratyphi C in Blood Specimens by Real Time Polymerase Chain Reaction Technique/Ghada Ahmed Mohammed Ali;Humodi Ahmed Saeed.-Khartoum:Sudan University of Science and Technology,college of Medical Laboratory Science,2008.-37p. : ill. ; 28cm.-M.Sc.
Abstract: A preliminary study was conducted during the period of March 2007 to March 2008 to detect S. paratyphi C directly in blood. Forty seven blood specimens were collected from patients suffering from enteric fever in Khartoum State. DNA of each specimen was extracted using phenol chloroform method. S. paratyphi C was detected by the aid of real time PCR using Quantica thermocycler. Only 1 (2.1%) of specimens was found positive and the rest 46 (97.9%) were negative. The study concluded that the real time PCR facilitates detection of S. paratyphi C without bacteriological culture and identification.
Description: Thesis
URI: http://repository.sustech.edu/handle/123456789/3385
Appears in Collections:Masters Dissertations : Medical Laboratory Science

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