Detection of Shigella bodyii in Stool Specimens by Real time Polymerase Chain Reaction

Abstract

This study was carried out in the period from May 2007- March 2008 to detect the presence of Shigella bodyii in patients with diarrheal disease. Fourty seven specimens were collected from patients attending Khartoum Teaching Hospital, Gaffer Ibniauff Specialized Hospital & Omdurman Teaching Hospital. Bacterial DNA was extracted from each stool specimen using phenol- chloroform technique. Then Real Time PCR technique was adopted to detect the presence of Shigella bodyii using specific primer. The result revealed that only 7 (14.8%) specimens were positive, while 40 (85.2%) were negatives specimens. The study concluded that the Real Time PCR facilitates detectio0n of Shigella bodyii without bacteriological culture. Comment on the number of the cell of Shigella bodyii according to cycle threshold shown by generated graphs. Show the distribution of the 7 positive specimens according to the hospital, gender & age group.