Detection of Enterobacter cloacae in Stool Specimens by Real-time Polymerase Chain Reaction

Abstract

This study was carried out during the period from May 2007 to March 2008 to detect the presence of Enterobacter cloacae in stool specimens from patients suffering from gastrointestinal disturbance. Forty six samples were collected from patients attended to Khartoum Teaching Hospital, Omdurman Teaching Hospital, Khartoum pediatrics Hospital and Omdurman pediatrics Hospital. DNA was extracted directly from stool specimens and used for the subsequent experiment. The forty six specimens were examined using Real time PCR Technique. The study revealed only seven (15c/o) specimens were positive and the remained specimens thirty nine (85%) were negative. The study concluded that the real-time PCR technique facilitates rapid detection of .bacterial pathogens directly from clinical specimen without culturing of bacteria