Detection of Klebsiella oxytoca in Sputum Specimens by Real-time Polymerase Chain Reaction Technique

Please use this identifier to cite or link to this item: https://repository.sustech.edu/handle/123456789/3325

Title:	Detection of Klebsiella oxytoca in Sputum Specimens by Real-time Polymerase Chain Reaction Technique
Authors:	Abd-alla, Shaza Mohammed Ahmed Supervisor,- Humodi A. Saeed
Keywords:	Klebsiella oxytoca Sputum-Specimens
Issue Date:	1-Jan-2008
Publisher:	Sudan University of Science and Technology
Citation:	Abd-alla,Shaza Mohammed Ahmed.Detection of Klebsiella oxytoca in Sputum Specimens by Real-time Polymerase Chain Reaction Technique/Shaza Mohammed Ahmed Abd-alla;Humodi A. Saeed.-Khartoum:Sudan University of Science and Technology,college of Medical laboratory Science,2008.-43p. : ill. ; 28cm.-M.Sc.
Abstract:	This study was carried out in the period from May 2007 - March 2008 to detect the presence of Klebsiella oxytoca in patients suffering from pneumonia. Forty six sputum specimens were collected from patients attending Albangadid Hospital, .Alshab Hospital, and National Health Laboratory .Bacterial DNA was extracted from each sputum specimen by phenol chloroform .Real Time PCR technique was adopted to detect the presence of Klebsiella oxytoca .The result revealed that only three (6.5%) specimens were positive The study concluded that the Real-time PCR has emerged as a valuable tool for the rapid testing of various biological specimens and body fluids for the presence of .micro organism
Description:	Thesis
URI:	http://repository.sustech.edu/handle/123456789/3325
Appears in Collections:	Masters Dissertations : Medical Laboratory Science

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