Detection of Enterobacter aerogenes in Urine Specimens By Real Time Polymerase Chain Reaction Technique

Abstract

This study was carried out during the period from May 2007- March 2008 to detect the presence of Enterobacter aerogenes in patients suffering from urinary tract infection. Ninty three samples were collected from patients attended Khartoum Teaching Hospital, Ibrahim Malik Teaching Hospital and Bashir Hospital. Bacterial DNA was extracted from each urine specimen using phenol choloroform technique. Real time PCR technique was adopted to detect presence of E. aerogenes. The result revealed that only one (1.1%) specimen was positive. The study concluded that the real time PCR technique facilitates detection of bacterial pathogens without bacteriological culture.