Detection of Escherichia coli in Urine Specimens by Real- time Polymerase Chain Reaction Technique

Abstract

This study had been carried out in Khartoum state during period between May 2007 to March 2008 to detect E.coli in patients with urinary tract infection. Forty six specimens of urine were collected from different hospital in Khartoum state. Bacterial DNA was extracted from each urine specimen by using phenol-chloroform method, then the real time PCR technique was adopted to detect E.coli. The study showed that the positive results were 45.7% and the negative results were 54.3%. The study concluded that the gene probe technique facilitates detection of bacterial pathogens without bacterial culture.