Demonstration of Prostatic Specific Antigen and Collagen Fibers in Tissue Affected with Prostatic Neoplasia and Nodular Hyperplasia using Immunohistochemical and Masson Trichrome Techniques

Abstract

This retrospective study was conducted at Soba University Hospital and Sudan University of Science and Technology during the period from April to September 2007, The study aimed to detect the presence of Prostatic Specific Antigen PSA, and to identify collagen fibers in tissue affected with prostate cancer and benign prostatic hyperplasia. 49 samples were selected and classified into four categories that were previously diagnosed as: ten samples were diagnosed as benign prostatic hyperplasia, three samples were diagnosed as well differentiated adenocarcinoma, eighteen samples were diagnosed as moderately differentiated adenocarcinoma, and eighteen samples were diagnosed as poorly differentiated adenocarcinoma, PSA was identified using immunohistochemical method, by using dako system (Invision) technique. This study concluded that there was association between PSA and prostatic cancer. Also Collagen fiber was identified using Masson Trichrome stain, The morphology of collagen fibers, show different characteristic feature according to the pathological process, accordingly it was sparse in amount in case of benign prostatic hyperplasia and was more dense in case of malignant cases more over the amount of fibers is increasing with grading of the tumors.