Detection of Citrobacter diversus in Urine Specimens by Real-Time Polymerase Chain Reaction Technique

Abstract

This study was carried out during the period from May 2007- March 2008 to detect the presence of Citrobacter diversus in patients suffering from urinary tract infection. Forty six samples were collected from patients attended Khartoum Teaching Hospital Bhri Teaching Hospital and National Health Laboratory. Bacterial DNA was extracted from each urine specimen using phenol chloroform technique. Real-time PCR technique was adopted to detect presence of C.diversus The result revealed that only three (6.5%) specimens were positive for C.diversus and 43 (93.5%) were Negative. The study concluded that the Real-time Polymerase chain reaction technique facilitates detection of bacterial pathogens without bacteriological culture.