Determination of the Pattern of Inheritance in Parents of Sickle Cell Anaemia Patients who referred to three hospitals in Khartoum state

Abstract

This was descriptive and cross-sectional study was carried out in Khartoum state during the period from November 2008 to January 2009. The study was designed to determine the pattern of inheritance of sickle cell anemia in parents of patients who referred to three hospital of Khartoum State. Hundred parents (50 mother and 50 father) were selected for this study, from Khartoum pediatric Hospitals, (2,5) ml of venous blood was taken from all parents and put it in an anticoagulant container. Complete blood count (CBC) was determined by use of Sysmex instrument N-50, and samples were investigated for HbS by use of sickling test. Hemoglobin electrophoresis was performed to determine the pattern of inheritance of sickle cell gene. The results were as followed: 97% of samples were heterozygous form of Hb S (ββ S) and the remaining were homozygous form of HbS (βSβS). 89% red cell morphology was normocytic normochromic cells, and the remaining were microcytic normo-hypochromic cells. The means of count and cell indices in the carrier and diseased parents as follows respectively: the mean of white blood cells count(TLC) was 8.5×10 9/l and 6.9×109/l , the mean of red blood cell count(RBC) was 4.6×1012/l and 3.9×1012/l, the mean of hemoglobin(Hb) was13g/dl and 9g/dl, th mean of hematocrit(PCV) was41.2% and39.3%, the mean of mean cell volume (MCV) was 86.2 and 86.3fl, the mean of mean cell hemoglobin(MCH) was30pg and 29.1pg the mean of mean cell hemoglobin concentration (MCHC) was 31.2% (58%) and31.3%, the mean of platelets count(PLT) was 207×103/μl and 298×103 /μl. The highest frequency of sickle cell anemia was found in Messaria tribe (40%), followed by Bargo tribe (30%), Hausa and Rezaigat (8%), Taisha and Jawama (6%), and Zagawa (2%).