Frequency and Antimicrobial Resistance of Shigella sonnei in Patients with Bacillary Dysentery

Abstract

This study was carried out in Khartoum state during the period October 2008_March 2009 to determine frequency of sonnei in patients Shigella with bacillary dysentery and its antimicrobial resistance. A total of 253 stool specimens were collected from patients to have bacillary attended Haj Elsafi hospital, Omdurman Teaching Hospital and Alban jaded hospital . The specimens were cultured in selnite F broth for overnight, and then subculture on deoxycholate to Xylose lysine isolate the causative agents. The identification of the isolated bacteria bacteria was done by colonial morphology, Gram stain and API 20 E system. Antimicrobial resistance of each isolate was determined by modified Kirby – Bauer disk diffusion method. E . test was used to determine minimum inhibitory concentration of antimicrobial agents. The result reveled that shigella sonnei was isolated from 5 specimens. Study on antimicrobial susceptibility test reveled that the resistance rate of Shigella sonnei was 100 % to ceftazidime and tetracycline, 0 % to ciprofloxacin, gentamicin and choloramphenicol. The minimum inhibitory concentration ( MIC ) of were 0.001μg to choloramphenicol, 0.5μg to tetracycline, 0.10 μg to gentamicin, 0.001μg to ciprofloxacin 0.01 μg to ceftazidime. The MIC50 and MIC90 of antibiotics were, 0.5μg each to tetracycline, 1.1 μg ciprofloxacin, 0.10 μg gentamicin, 0.001 μg choloraphenicol, and the MIC50 of ceftazidime was 0.01 μg and the MIC90 of ceftazidime was 0.1 μg. The study concluded sonnei is slighty high. that the presence of Shigella The antimicrobial resistance for Shigella sonnei was high too.