Isolation and Identification of Uncommon Enterobacterial Species from clinical specimens

Abstract

This study was carried out in Khartoum state during the period from November 2008 to April 2009, to isolate and identify uncommon enterobacterial species from clinical specimens. Three hundred and eleven urine specimens, one hundred and twelve wound specimens, and three hundred and eight stool specimens were collected from patients attended Khartoum Teaching hospital, Gaffer Iben Auff specialize Hospital for children, and Omdurman Teaching Hospital. The urine and wound swabs were cultured on blood agar and MacConkey's agar for primary isolation of pathogens. Stool specimens were cultured in selenite F broth and incubated. Then subcultured on xylose lysine deoxycholate agar. Identification of the isolates was done by , colonial morphology, Gram s stain and biochemical tests using API 20 E. Modified Kirby-Bauer disc diffusion method was adopted to determine the resistance of the isolates to some antimicrobial agents. Minimum inhibitory Concentration (MIC) of chloramphenicol, ciprofloxacin, tetracycline, gentamicin, ceftazidime, amikacin, ceftriaxone, tecarcyline, amoxicillin, amoxyclav, nalidixic nitrofuratoin, acid, cephotaxime, determined by E. test. and co-trimoxazole, tobramycin were Out of 731 specimens investigated, 5 uncommon enterobactrical species were recovered. These isolates were Salmonella choler.arizonae, chroactrum anthropi, Enterobacter intermedius, Citrobacter youngae, and Pasturella multocida . The majority of the isolate were resistant to antimicrobical agents. The study concluded that uncommon enterobactrical species exist in clinical specimens.