Determination of Fibrin D-dimer level in Patients with Sickle Cell Disease in EL-Gadarif state

Abstract

This was comparative, cross-sectional and analytical study has been carried out in Algadarif state from May to August 2009. The study aimed to determine the level of D- dimer inorder to detect the significance of activated fibrinolysis in sickler patients randomly (steady state and non steady state). Sixty one blood samples were collected into trisodium citrated containers (2.5 ml) from sickler patients in steady state or crises. Fifteen control samples were collected from healthy people matched with age and sex of patients. All samples were investigated for PT, PTT, and INR was calculated using full automatic coagulation analyzer CA-500, D-dimer was investigated using NycoCard D-dimer Single Test. Data were analyzed using program of Statistical Package for Social Sciences (SPSS). The study revealed that the mean level of D-dimer was higher in all sickler patients (whether in steady state or in non steady state), and the P value was (0.000) when compared with control. But (P values > 0.005) in case of PT, PTT&INR and this means the differences were not significant. Also the study revealed that no significant difference between patients in steady state and non steady state for the parameters of PTT and D-dimer (p 0.736 and 0.721 respectively) ,while significant difference was found in PT&INR(P 0.004 and .003 respectively). The study concludes that the activation of fibrinolytic process does not associate with patient clinical state, but associated with inheritance of Sickle Cell Disease .PT& INR values can be effective in follow up of sickler patient’s thrombotic crises. Very important recommendation that more studies on larger number of patients must be done to detect the invivo cause of fibrinolysis activation, and to detect the diagnostic test for crises.