Frequency and Antimicrobial Resistance of Proteus vulgaris in Diabetic Patients with Septic Wounds

Abstract

This study was carried out in Khartoum State during the period from November 2008 to March 2009, to determine the frequency and antimicrobial resistance of P. vulgaris in diabetic patients with septic wounds. One hundred and twelve wound swabs were collected from patients attended Mac Nimir Complex for Diabetic and Surgery, Bashair hospital, and Jabir Abuizz Diabetic Centre. The specimens were cultured on blood and MacConkey's agars for primary isolation of the pathogen. Identification of the isolates was done by colonial morphology, gram’s stain and biochemical tests using API 20 E . Out of the one hundred and twelve wound swabs examined, 6 (5.4 %) P. vulgaris were recovered. Modified Kirby-Bauer disc diffusion method was adopted to determine the resistance rate of P. vulgaris to amoxycillin, ceftriaxone, ticarcillin, ciprofloxacin and amikacin. The result revealed that the antimicrobial resistance of P. vulgaris was as follows, amoxycillin (100 %), ceftriaxon (33.3 %), ciprofloxacin (0%), ticarcillin (0%) and amikacin (0 %). Minimum inhibitory concentrations (MICs) for amoxicillin and ceftriaxon were determine by E. test. The result indicated that the MIC, MIC50 and MIC90 of amoxicillin was (>240 μg/ml ), and ceftrixone were (0.01 and 0.1 μg/ml). The study concluded the frequency of P. vulgaris in diabetic septic wounds was slightly high. The antimicrobial resistance to amoxicillin was high.