Phytochemical Investigation of The Leaves And Stems of Hapophyllum tuberculatum

Abstract

The objective of this study is to extract the alkaloids from Alhazza shrub “haplophyllum tuberculatum” which grows in Sudan, and to determine the structure of the extracted alkaloids. Alhazza samples were collected from Aljazeera Slang (25kilometre north of Khartoum) . The samples were prepared for analysis, by separating leaves from stem, drying them in an open area in a well ventilated room, crushing them into small pieces and then grinding them to fine powder. Extraction was done using soxhlet with methanol as solvent. The methanol extract was defatted to remove waxes, gums, and chlorophyll using hexane as defatting solvent. The defatted methanol extract was further extracted by chloroform to separate the alkaloidal constituents of the plant. Identification of the presence of the alkaloids was made by Mayer's reagent and Dragendorff's reagent and both gave positive results. The alkaloidal constituent was separated by thin layer chromatography using petroleum ether : ethyl acetate 2:3 as solvent system .The extracted alkaloids were named as compound1and compound2. Spectral analysis UV, IR, 1HNMR, MS of compound1 and compound2 was done by UV, IR, 1HNMR, MS spectrophotometers and the following structures were proposed .