Frequency of Providencia stuartii in Clinical Specimens and its Antimicrobial Resistance

Abstract

This study was carried out in Khartoum state during the period from November 2008 to March 2009, to determine frequency of P. stuartii in clinical specimens and its antimicrobial resistance. Four hundred and twenty three urine and wound swabs (two specimens from urine and three specimens from wound swabs) specimens were collected from patients attended Khartoum Teaching Hospital, Gaffer Iben Auff specialized Hospital for Children, Fedial Medical Center and Omdurman Teaching Hospital. The specimens were cultured on blood agar and MacConkey`s agar for primary isolation pathogen . Identification of the isolates was done by colonical morphology, gram stain and biochemical tests using API 20E. Modified Kirby – Bauer disc diffusion method was adopted to detect the resistance rate of P. stuartii to nitrofuration, amoxycillin, nalidixic acid, co-trimoxzole, amoxyclav, ticaricillin, ceftriaxone, ciprofloxacin and amikacin. Minimum inhibitory concentration (MICs) to all antimicrobial agents were determine by E.test. Of the four hundred and twenty three urine and swab specimens examined, only 5(1.9%) P. stuartii were recovered. The result revealed that the antimicrobial resistance of P. stuartii was as follows; nitrofuration, amoxycillin, ticaricillin were (100% each) and nalidixic acid, co-trimoxazole, amoxyclav, ceftriaxone, ciprofloxacin and amikacin were (0% each). The result indicated that the MIC, MIC50 and MIC90 of nitrofuration (>240mg/ml each), co-trimoxzole (0.5 mg/ml each), amoxycillin (5- 120mg/ml, 60 mg/ml and 20 mg/ml), amoxyclav (30 mg/ml each), nalidixic acid (0.05mg/ml each), amikacin (0.1-0.5mg/ml,0.5mg/ml each) ciprofloxacin (7.5mg/ml each), ceftriaxone (0.25mg/ml each) and ticaricillin (30-60 mg/ml and 60mg/ml each). The study concluded that P. stuartii is one of the causative agent of urinary tract infection and wounds infections.