Synthesis of Molecular Imprinted Polymers for Assay of Non-Steroidal Anti-inflammatory Drugs Using Different Functional Monomers

Abstract

The aim of this study was prepare MIPs using Ibuprofen and diclofenac sodium as templates Three MIPs prepared of Ibuprofen using 1-vinylimidazole (1-VI) , 2-hydroxy ethyl metha acrylate (2-HEMA) and Styrene as functional monomers, as well as Ethylene glycol dimethacrylate (EGDMA) and N-N methylene bis acrylamide as cross linkers and benzoyl peroxide (BPO)as an initiator,. In addition, three MIPs were prepared from Diclofenec sodium using on1-vinylimidazole (1-VI), acrylamide (AA) and Styrene as active monomers. Ethylene glycol dimethacrylate (EGDMA) as cross linker and benzoyl peroxide (BPO)as initiator . The parameters studied to detect Ibuprofen and diclofenac sodium included concentration range, slope, detection limit, response time, life time, correlation coefficient and the working pH range, Interferences were also studied selectivity against(K+,Ca+2,Al+3, TSC, MP,PP) Five membranes were prepared from MIP for IBP (IBP-MIPs)two each for MIP1and MIP3 and one from MIP2 The membrane were prepared by mixing an appropriate plasticizers Dioctyl phthalate (DOPH), Nitro benzene (NB), Tri tolyl phosphate (TTP) , Dibutyle phthalate (DBPH),and Dibutyle Sebacate (DBS) with IBP and DFS (as template) in PVC matrix. The membrane electrodes for IBP-MIP1+DOPh , IBP-MIP1+NB, IBP-MIP2 + TTP , IBP-MIP3+DBPH and IBP-MIP3+DBS gave the following results: slops (30.5, 29.9, 19.04, 19.003, 20.46 )mV/decade respectively ,linear concentration range(10-6-10-1)M, detection limits (1.2×10-7, 2.3×10-8 ,1.86×10-7 ,7×10-7and 7.1×10-7) M respectively ,correlation coefficient (0.9996 ,0.9996 ,0.9999 0.9996 and 0.9995) respectively and life time (45, 12, 25,40 and 30) days respectively the working pH was studied for IBP electrodes and it was effective in the range (1 to 9±1) Three membranes(MIPs),however were prepared from Diclofenac sodium (DFS) one each of MIP1,MIP2 and MIP3 DFS membranes DFS-MIP1+TEHP, DFS-MIP2+DBPH , and DFS-MIP3+DOPH gave results of showed that slopes (17.87,19.415 and 19.168) mV/decade, linear concentration (10-6-10-1)M, detection limits (7×10-6,2.9×10-7, and 4.5×10-7 ) M ,correlation coefficient (0.9997 ,0.9998 , and 0.9996) and life time (37 , 38, and37)days respectively the working pH was studied for DFS electrodes and was effective in the range (1 to 9±1) These ISEs have been tested in the detection of of Ibuprofen and diclofenac sodium pharmaceuticals using direct method (DM).standard addition (SAM), and multiple standard addition (MSA) method as well the separate solution method was used to calculatc the selectivity coefficient .The accuracy and precision were examined and calculating recovery Rec.% and relative standard deviation RSD% , respectively for The eight ISEs.