Molecular Detection of Extended- spectrum Beta-lactamase Genes in Proteus vulgaris

Abstract

Members of the family enterobacteriaceae- producing ESBLs constitute a serious threat to current ß - lactam therapy. Moreover, in vitro detection of ESBLs expression has proved to be difficult because many of these strains are reported susceptible to the widely used and broad-spectrum ß-lactam antibiotics. This study was carried out in Research Laboratory of Sudan University of Science and Technology during the period from December 2009 to May 2010, to detect TEM, SHV and CTX-M genes in ESBLs-producing Proteus vulgaris Six strains of Proteus vulgaris were obtained from the Research Laboratory . The strains were cultured on nutrient agar and examined microscopically to check their purity. DNA extracted from each strain by boiling method. Multiplex PCR method was adopted to detect gene present in Proteus vulgaris. The results showed that only TEM gene existed in all tested strains. It is concluded the TEM is the common gene present in Proteus vulgaris. further studies with large number of samples are required to validate the present result .