Molecular Identification of Human Papilloma Viruses 16 and 18 among sudaneses patiants with oral lesions

Abstract

This is a retrospective study conducted in Radiation Isotope centre-Khartoum during December 2009 - April 2010. The study aimed to investigate Human Papilloma Virus (HPV) in oral tumor patients were referred to Radiation Isotope centre-Khartoum. HPV was investigate among 55 patients aged between 31-71 years (40 were oral squamous cell carcinoma (OSCC) and 15 were benign lesion). The DNA was extected from tissue sample then polymerase Chain Reaction (PCR) applied to investigate the HPV. In this study, among 40 patients suffering from OSCC, HPV was identified in 6 patients (10% of patients were males and 5% were females). Among HPV positive patients, 2 (5%) were diagnosed as HPV type (16), and 4 (10%) were diagnosed HPV type (18). However, patients with benign lesion were HPV negative. Distribution of OSCC patients was recorded according to the patient’s residency as follow; 4 patients with OSCC from Khartoum, 1 from the centre of Sudan, 1 from the east, 10 were from west, 6 were from the south, and 18 were from the northern Sudan. In contrast, patients with benign oral lesions, 4 patients were from Khartoum, 1 from the centre, 1 from the west, 3 were from the south, and 6 were from north of Sudan. The OSS patients were diagnosed according to the pathology as fallow; 16 males and 4 females were investigated with well differentiated squamous cell carcinoma, 9 males and 4 females were with moderate differentiated squamous cell carcinoma, and 3 males and 4 females with poor differentiated squamous cell carcinoma. However, 13 males and 2 females were investigated benign oral lesion. Eventually, the existing scientific data continue to suggest that infection of HPV plays a critical role in the development of OSCC, perhaps in conjunction with other risk factors. The study recommended screening more patients who suffer from OSCC using more advanced molecular techniques to confirm the role of the HPV in developing of OSCC in Sudan.