Molecular Detection of Extended-Spectrum Beta-Lactamaes Genes in Klebsiella oxytoca Isolated from Clinical Specimens

Abstract

Beta- lactamases continue to be the leading cause of resistance to β-lactam antibiotics among gram-negative bacteria. In recent years there has been an increased incidence and prevalence of Extended-spectrum lactamases (ESBLs).This study was conducted in the Research Laboratory in Sudan University of Science and Technology. The study was carried out during the period from December 2009 to May 2010, to detect TEM, SHV and CTX-M genes in ESBLs-produsing Klebsiella oxytoca. The Klebseiella oxytoca strains were obtained from the Research Laboratory. All strains were checked for purity by sub-culturing on nutrient agar and examined microscopically. Bacterial DNA was extracted from each isolate using boiling method. Multiplex Polymerase chain reaction (PCR) was adopted to detect these genes. The result revealed presence of TEM gene in nine isolates. It is concluded that, TEM gene is the commonest gene in K. oxytoca isolates. Further studies with large number of bacterial isolates are required to validate this results.