Identification of Some Carcinogenic Compounds in Nicotiana rustica (Toombak) leaves

Abstract

Tobacco used for manufacturing of toombak is species of Nicotiana rustica leaves. Tobacco ( Nicotiana rustica) contains carcinogenic N –nitrosamines. these are formed by N-nitrosation of the major tobacco alkaloids( nicotine and nor nicotine) during curing and fermentation of tobacco . Among the N–nitrosamines formed during curing ( processing) of tobacco : 4-(methylnitrosoamino)-1(3-pyridyl)-1-butanone (NNK) and Nnitrosonornicotine (NNN) . This study was conducted to Identify of carcinogenic N –nitrosamines (NNN and NNK) samples of Sudanese toombak were collected from five vendors in Bahri . Separated funnel was used to extract N-nitrosamines from the samples. GC-MS Spectrometry and Thin-Layer Chromatography (TLC) were used to detect and the identify Nnitrosamines extract. It was noted that, all the above carcinogenic compounds were detected in toombak