Molecular Detection of Extended- spectrum Beta-Lactamase Genes in Enterobacter sakazakii Isolated from Clinical Specimens

Abstract

This study was carried out in Research Laboratory of Sudan University of Science and Technology during the period between December 2009 to May 2010, to detect extended spectrum beta-lactamase genes in Enterobacter sakazakii Six bacterial strains were obtained from Research Lab. DNA was extracted from each strain using boiling method. Purity of DNA was conducted by run the extract on 1.5% agarose gel. Multiplex PCR technique was adopted to detect bla TEM, bla SHV and bla CTXM genes. This study showed that 6 strains (100%) of Enterobacter sakazakii were positive for bla TEM gene and the other genes were not detected. .The study concluded that TEM gene is responsible for ESPL in E. sakazakii strains