Detection and susceptibility testing of Aspergillus spp from sputum of Asthmatic and Cystic fibrosis patients in Khartoum State

Abstract

This study was a cross-sectional case study, conducted during the period from February to May 2018 at El Shaab Teaching Hospital. The practical part was carried out in Research laboratory of medical laboratory science collage, Sudan University of Science and Technology. The main objective of this study was to study allergic bronchopulmonary aspergillosis in asthmatic and cystic fibrosis patient and found an effective treatment of it in Khartoum State. A total of 100 sputum specimens were collected from asthmatic (75) and cystic fibrosis patients (25), 43 samples from male and 57 from female. The sputum specimens were processed for isolation of Aspergillus species by standard method. Aspergillus species were isolated from 5(5%) out of 100 sputum sample. All positive Aspergillus species samples processed for estimation of MIC of voriconazole and itraconazole by E. test. There were no significant association between the prevalence rate of ABPA in Asthmatic and Cystic fibrosis disease (P value=0.42). This study concluded that, the frequency of ABPA in Asthmatic (4%) and cystic fibrosis patient (8%) represent (5%) from all sample and voriconazole is more effective than itraconazole against Aspergillus.