Molecular Detection of Helicobacter pylori Virulence Genes in Patients with Gastritis, Gastrodudenities and Peptic Ulcer in Khartoum State

Abstract

Helicobacter pylori is a Gram negative, microareophilic bacteria usually found in the stomach, produce urease, oxidase,hydrogenase and catalase enzymes ; cause very harmful damage to the stomach epithelial cells by different mechanisms and this harmful expressed by human body as gastritis (acute or chronic), duodenal ulcer ,peptic ulcer and Helicobacter pylori may extend to cause mucosa associated lymphoid tissue lymphoma and cancer. The aim of this study was to detect Helicobacter pylori in Gastric and Duodenal biopsies from symptomatic patients by using urea A gene specific primer and the frequency of Helicobacter pylori virulence genes IceA1, IceA2 , glmM and HPU genes among Sudanese patients attending to Fedil special hospital in Khartoum by using polymerase chain reaction assay. Participants of this study were of different age groups, different signs and symptoms and different life habits whose visited Fedail special hospital in Khartoum state at gastric endoscopic unit. Total number of 61 gastric biopsy samples were collected from antrum region of the stomach by gastro endoscopist .There were 50(82%) of all isolated biopsies positive for Helicobacter pylori urea A gene, those positive urea A gene isolates were analyzed for detection the frequency of the virulence genes, Most of study population were females26(52%) compare to males 24(48%)and the frequency of each virulence genes were IceA1 8(16%),IceA2 31(62%),gLmM 7(14%) and4 (8%)for HPU and the main virulence factors in all isolated biopsies are IceA2 gene.This study clarified that IceA2 gene is the common isolated gene by 31(62%) compared to other virulent genes.