Molecular Detection of Helicobacter pylori UreC and 16sRNA genes in Saliva from Stool Antigen Positive Patients in Saad Rashwan Medical Center in Khartoum State (2019)

Abstract

Helicobacter pylori infection is responsible for several gastric disease especially major ulcers in the stomach and small intestine, transmission mechanism of H. pylori are still unclear but the fecal oral route might be the most important, and the oral cavity can be an extra gastric reservoir for the organism. This is a cross sectional study conducted to detect H. pylori( UreC, 16sRNA ) genes from saliva of stool antigen positive patients in Saad Rashwan medical center from March 2018 to March 2019. Total of fifty ( N=50 ) saliva specimens were collected from patients ( males and females of different age). The DNA was extracted from saliva samples using quinidine chloride method and 16sRNA, UreC (glmM) genes were used in multiplex polymerase chain reaction to detect H. pylori. Out of 50 saliva samples collected in this study one sample (2%) was found positive for Helicobacter pylori (UreC gene), and all other samples were found negative. This study conclude that H. pylori can be found in saliva specimens, which can be a source of transmission.