Antimicrobial Activity of Solenstemma argel Extract against Selected Uropathogenic Isolates In Khartoum State

Abstract

An alarming increase in bacterial strains resistant to existing antibacterial agents demands a renewed effort to seek agents effective against pathogenic bacteria resistant to antibacterial. The aim of this study was to study the antibacterial activity of different concentration of methanol extracts of medicinal plant Solenstemma argel using cup-plate agar diffusion method on selected uropathogenic isolates bacteria. The study was conducted in Khartoum State, Sudan between February and April 2017. One hundred selected uropathogenic bacterial clinical isolates were used in this study. The most frequent of the isolates were E.coli 60(60%), followed byK.Pneumonia7(7%),P.mirabilis10(10%),Ps.aeruginosa 10(10%),S.aureus 8(8%), and E.faecalis 5(5%). The methanol extract of Solenstemma argel was screened for it is antimicrobial activity against uropathogenic clinical isolates. Which showed antimicrobial activity against both Gram positive and Gram negative bacteria. It was clear that the most powerful effect was observed in case of E.faecalis; moderate action against E.coli; K.pneumoniae; P.mirabilis;S.aureus; and low effect against Ps.aeruginosa. The clinical isolates of E.faecalis, E. coli , S. aureus and Ps. aeruginosa was sensitive to all concentrations of Solenstemma argel extract. The clinical isolates of K. pneumoniae and P.mirabilis was sensitive to 100% (w/v) and 50%(w/v) of Solenstemma argel extract but resistant to 25%(w/v) concentration.