Molecular Detection of Helicobacter pylori GLmM Gene among Gastritis and Duodenitis Patients in Albogaa Specialized Hospital - Omdurman

Abstract

Helicopacter pylori has a significant pathogenic role in gastritis and peptic ulcer disease, as well as in gastric adenocarcinoma, mucosa-associated lymphoid tissue (MALTS) lymphoma and possibly cardiovascular disease. This study aimed to detect Helicobacter pylori GlmM gene by using polymerase chain reaction (PCR), in patients with gastritis and duodenitis attending to the Albogaa specialize Hospital in Omdurman City during the period from April 2018 to January, 2019. A total of 50 patients (n=50) of both gender and ages ranging from 19 to 81 years old were included in this descriptive cross-sectional hospital based study. Endoscopy biopsy samples were collected. Specimens were preserved and transported in phosphate buffer saline. Genomic DNA was extracted using Guanidine hydrochloride protocol and examined for Helicobacter pylori GLmM gene by PCR technique. Amplified PCR products were separated by agarose gel electrophoresis. The gel was viewed and photographed on Gel-Documentation System. Out of the 50 samples examined, 9 (18%) were positive for Helicobacter pylori GlmM gene, out of them: 3 (12%) were from male and 6 (24%) from female. 7 (21.2%) of them have gastritis, 1 (20%) of them have gastric ulcer, 1 (12.5%) of them have other diagnosis. In patients age group less than 30 years, 5 (31.3%) were positive for Helicobacter pylori GLmM gene, 30-60 years 2 (14.3%) were positive and age group more than 60 years 2 (11.1%) of them were positive gene detection. The percentage of H. pylori detected by GlmM gene is low when compared to other studies. The finding of this study indicates that there was no significant association between gastritis and duodenitis with H. pylori infection