Immunohistochemical Detection of CDX2 in Colorectal Cancer among Sudanese Patients

Abstract

This hospital based analytical retrospective case control study conducted at Ibn Sina hospital, National Public Health Laboratory (Stack) and Radiation and Isotropes Center Khartoum (RICK (and Sudan University of Science and Technology- College of Medical Laboratory Science during the period from March to July 2018. The study aimed to detect the CDX2 expression in colorectal tumors using immunohistochemistry. Forty paraffin blocks were collected from patients previously diagnosed as colorectal tumors. 25(62.5%) samples were malignant and the remaing 15(37.5%) samples were benign. The paraffin blocks were cut by rotary microtome then stained by immunohistochemistry method (new indirect Dako technique). The data obtained was analyzed using SPSS program version 11.5. Mean, frequency and chi square test were calculated. The age of patients ranged between 32 to 85 years with mean age of 61 years. The study revealed that most of patients were more than 50 years representing (77.5%) 31 and the remaining 9 (22.5%) were less than 50 years. The majority of the patients were males represented (60%) 24 and the remaining (40 %) 16 patients were females. CDX2 expression showed positive result among malignant colorectal tumors in 5(12.5%) samples and negative expression in 20(50%) samples, while benign colorectal tumors showed positive result in 13(13%) samples and negative expression in 2(50%) samples. This result showed significant statistical association(P value 0.000). The study concluded that the CDX2 expression is associated with benign colorectal tumors.