Detection of Different types of Mucins in Adenocarcinoma of the Large Bowel Using Special Stains

Abstract

This retrospective study was conducted in histopathology laboratories in Bahry hospital and fideal hospital, and laboratory of Dr. Ishraga Ahamed Fargalla, during the period from This study aimed to detect the April to August 2011. different adenocarcinoma of the large types of mucins in bowel. 67specimens from adenocarcinoma of large bowel were stained by six histopathological special stains to detect types of mucins and to correlate histological types and grade of the tumor with mucin type.the Haematoxylin and Eosin (H&E) for general morphology, and periodic acid schiff's reagent for neutral mucin and N-acetyl sialomucin(enzyme labile),variable for sulfated and negative for O-acetylsialomucin(enzyme resistant),and Phenylhydrazine-PAS stain which is negative for neutral mucin and positive for other periodate-reactive mucins, and combined alcian blue- PAS stain which is differentiated between acid and neutral mucin, and combined aldehyde fuchsin-alcian blue stain to differentiate between sulfated and carboxylated mucin, and alcian blue stain PH1for weakly sulfated mucin. The study concluded that: The type of mucin found in this study, carboxylated sialomucin Oacetyle was the majority type 22 (32.80%), followed by carboxylated sialomucin N acetyl form 19 (28.40%), neutral mucin and carboxylated sialomucin were found in 12 (17.90%), and neutral and sulfated mucin in 6 (9.0%), while sulfated mucin in 4 cases(6.0%) and neutral and acid mucin in 4 cases also.The histological types of tumor are mucinous adenocarcinoma, non mucinous adenocarcinoma and signet ring type, distributed as 9(13.4%), 52(77.6%), 6(9.0%) respectively. The histological grade of tumor distributed as well differentiated 17(25.40%), moderately differentiated 38(56.70%) and poorly differentiated 12(17.90%). Finally we recommended conducting new studies contributing larger sample size ‫‪and using more specific and sensitive techniques to identify different‬‬ ‫.‪types and suptypes of mucins‬‬