Measurement of Hematological Parameters and Iron Status of Visceral Leishmaniasis in Children Attending EL –Gadarif Teaching Hospital

Abstract

This is an analytical case –control study, conducted at Al–Gdarif teaching hospital, during the period from January to April 2011. The aim of this study was assessment of the hematological parameters (complete blood count), and serum ferritin, tests in visceral Leishmaniasis (v l) patients, attended Al-Gdarif teaching hospital. Fifty visceral Leishmaniasis patients were informed about the study and agreed for participation .The study population (both sex) was divided into three group according to age . Five ml of venous blood was bleed ,from both patients and control , 2.5ml in EDTA( 1.2mg/ml) containers and 2.5ml without anticoagulant (serum) ,to investigated the CBC ,peripheral blood film ,reticulocyte count, and serum-ferritin tests .Fully automated hematological analyzer sysmex was used for CBC, and manual analysis for blood film ,and reticulocyte count ,and a fully automated immunoassay system (AIA -600 11) was used for Serum-ferritin analysis. The staining films( Ral-stain), were examined to detect the peripheral blood morphology, and other staining films(vital stain) to count reticulocyte cell manually . All visceral Leishmaniasis patients had mean value of HB( 7.2g/dl) ,RBC(3.6×106), Hct( 25.4%), MCV(69.5 fl), MCH(19.9 pg), MCHC(28.1g/dl), WBCs(3.2×106μl), Neutrophil(33.2%) ,And platelet(142.2×10 3μl), decrease, highly significant than control mean values( All p value=0.00).The mean of lymphocyte, and eosinophil,( 50.8% ,11.2%,respectively), significant increase than control mean values (p value=0.00) due to infecation. The mean of the Serum-ferritin in test (33.2 ng/ml), showed significant decrease than control (52.7 ng/ml) ,but with normal rang .The reticulocyte count showed significant increase in test ,than control (mean=4.8%).