Protein Profile of Helicobacter Pylori Isolates from Sudanese Patients with Gastroenterological Inflammation

Abstract

As chornic gastritis and peptic ulcer diseases, particularly duodenal ulcer, are the most common endoscopic findings in Sudanese dyspeptic patients, it was necessary to study the association between H. pylori infection and gastro- duodenal diseases in developing countries such as Sudan. Material and Methods: One hundred and twenty subjects referred to Gastroentrology Department, Ibn Sina Hospital, Khartoum were studied. Most of the patients were referred because of dyspepsia. After endoscopic examination 97 were found to have definite pathology and 23 were found to have normal endoscopic examination. Age range from 18 to 80 years old. . Male to female e sex ratio was (2:1). Endoscopic biopsies were collected from all subjects. In this study, culture of H. pylori was used as the gold standard method to which histological (urease) .test, was compared as alternative diagnostic technique Results:. 93 subject were H. pylori positive when using culture. The CLO test detected 85 H. pylori positive and 35 were negative . H. pylori was found not to be associated (p>0.05) with age, sex, cigarette smoking urease test, socio-economic status, residence, Tribe and water resources disposable system However, it is assocciated (p< 0.05) with complaints (epigastric pain, abdominal pain and vomiting), endoscopy finding (gastric ulcer, duodenal ulcer, gastritis and duodenitis) and the presence of 120 kda. The protein pattern of H. pylori when analyzed with sodium deodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) technique are not markedly different between strains of different origin SDS-PAGE The major protein bands with molecular weights of 120, 94, 88,75, 69, 67, 61, 48, 45, 42, 30,and 25Kda were variably present. IV The data from this study proves the high correlation of 120 Kda protein to endoscopic findings (x2 = 16.047 p value = 0.003 p < 0.05 highly significant. In this study 57 (61%) out 93 H. pylori strains that were isolated from 120 subjects possess the gene that expressed its 120 Kda product. The 120Kda was detected in H. pylori isolated from one subject with normal endoscopy , 11(11.8%) with duodenal ulcer, 8( 8.6) patient with gastric ulcer, 23(23.7%) patient with gastritis, 14 (15.1%) patients with duodenitis and only 1 patient with oesophagitis The present study confirms a high prevalence of H. pylori infection in Sudanese patients as in most population in Africa and other developing countries.