Detection of Human Papilloma Virus Genotype-51 among Cervical Cancer Patients Using PCR Technique in Khartoum State

Abstract

This is a descriptive, retrospective case study which was conducted at research laboratory in Sudan University of Science and Technology department of histopathology during the period from February to August 2017. The study aimed to detect the HPV-51 and cervical cancer patients using PCR technique, There by 40 formalin fixed embed tissue blocks of different cervical cancer type were collected. The tissue blocks were cut by Rotary microtome, prepared for DNA Extraction and then the viral DNA was detecting using PCR and a garose gel electrophoresis, the Data was analyzed manually, frequencies and percentage were calculated. The ages of patient were range between 30 to 70 years with mean age 50, Most of patient were more than 40 years representing 35/40 (88%) and the remaining less than 40 is 5/40 (12%). The study showed that keratin cervical cancer represents 87% and Non-keratin is 13%. The results of the DNA polymerase chain reaction of the human Papilloma virus were negative in all cervical tissue samples. The study concluded that HPV-51 does not play a role in causing cervical cancer.