Detection of Glucose-6-PhosphateDehydrogenase Deficiency in Neonates with Jaundice at Khalifa Hospital, Ajman, United Arab Emirates

Abstract

Glucose 6-phosphate dehydrogenase (G6PD) is an enzyme which is catalyzed the first step in hexomonophasphate pathway of glucose metabolism. G6PD is the most common hemolytic X-linked genetic disease. This was cross sectional study conducted at Sheikh Khalifah Hospital, Ajman – United Arab Emirates. During the period January-2015 to deceber-2015 which is aimed to detection G6PD among neonates with jaundice and to find possible correlation between G6PD and Homological test (CBC, DAT,Retic) And CRP as inflammatory parameter and total bilirubin as indicator of jaundice. Sample collected in EDTA and heparinized tubes for investigations and the results analyzed using SPSS version (2015). The present study shows that from 461 neonates participate in the study after approval from parents. (249 / 461), (54%) were male, while female were (212/461) (46 %) G6PD deficiency found in 40 neonate (40/461) (8.6%) and male were more affected compared with female. (28/40)(70%), (12/40) (30%). Other findings includes Hb in g/dl was 6-22 with avarage14g/dl, regardless to the positively or negatively towards G6PD deficiency. Reticulocytes count average from lowest count (0.6 to 7.0) Total WBC was estimated ration from low count (3.000 to 20.000) in cases deficient for G6PD, however, for the other cases is markedly variable from (3.000 to 111.000) which was not noted among deficient cases. About the direct agglutination test (DAT) there were only two positive cases 5% the rest were negative 95% in G6PD deficient neonates. However the same result in the rest 421 cases. CRP showed extremely variation in results. The lower estimates low 0.1 to 20 mg/L Total bilirubin: results showed frequently comprises from (87 to 375mmol/L) among deficient cases While among other hemolytic cases the results also look the same from less (40 to 400). Thisstudy supports the importance of implementing G6PD screening for neonates as there are no laboratory parameters specific for indicating G6PD deficiency. However, the importance of G6PD screening lies in the prevention of future hemolysis and complications.