Detection of Human Papilloma Virus Gentotype - 68 Among Cervical Cancer Patients UsingPCR Technique in Khartoum State

Abstract

This is a descriptive, retrospective case study conducted at research lab inSudan University of Science and Technology,department of histopathology during the period from January to September 2017The study aimed to assess the association between HPV-68 infection and cervical cancer among Sudanese patients through detection viral DNA using PCR technique, there by 40 formalin fixed embed tissue blocks of different cervical cancer type were collected. The tissue blockswere cut byRotary microtome, prepared for DNA Extraction and then the viral DNA was detecting using PCR and agarose gel electrophoresis, TheData was analysis manually, frequencies and percentage were calculated. The study includes 40 women their ages ranged between 30 to 70 years with mean age 50, Most of patient were more than 40 years representing 35/40 (88%) and the remaining less than 40 is 5/40 (12%). The study showed that keratinized cervical cancer represents 87% and non-keratinized is 13%. The results of the DNA polymerase chain reaction of the human papillomavirus-68 were negativein all cervical tissue samples. The study concluded that HPV-68 does not play a role in causing cervical cancer.