Physicochemical study of crude Gum and Foam Fractionated from AnogeissusLeiocarpus (Sahab)

Abstract

The gum fromAnogeissusLeiocarpus was fractionated into foam fraction A(hydrophobic) and B fraction( hydrophilic) and the percent yields were 14.6% and 85.1% respectively. Crude gum and fractions were analyzed using standerdmethods.The results have shown that moisture, ash, PH, nitrogen content, protein content and specific optical rotation, were 7.9%, 2.53%, 4.1, 0.392, 2.450 and -37.4 for crude gum then 8.7%, 2.51%, 5.17, 1.022, 6.39 and -38.7 for fraction A(foam/hydrophobic) and 11.3%, 2.54%, 4.8, 0.630, 3.94 and -35.2 for fraction B (hydrophilic) respectively. Cationic composition of the fractions in ppm were Ca( 522.9), K(262.5), Mg(38.4), Na(8.74), Zn(0.093) and Pb(0.124) for fraction A and for fraction B Ca(192.5), K(252.8), Mg(34.86), Na(11.71), Zn(0.093) and Pb(0.052). The study has shown closely similar results for the percentage ofuronic acid percent between crude gum(14.7%) and fraction B(12.1%) but fraction A(3.23%) has shown significantly different value. The intrinsic viscosity in g/cm3 for fraction B(0.65) was approximately three times greater fraction A(0.24) and double the value of the crude gum(0.43). In addition the value of intrinsic viscosity of the crude gum was found to be double that of fraction A. This sugar composition of the crude gum sample was found to be: Rhamnose(2.31%), Xylose(4.94%), Arabinose(34.95%) and Galactose(47.67%). In case of fraction A it was found to be Xylose(6.51%)Rhamnose(6.51%), Arabinose(36.3%) and Galactose(36.3%) while fraction B consists of Rhamnose(10.5%), Xylose(6.84%), Arabinose(27.35%) and Galactose(40.12%).