Evaluation of Hematological Parameters of Sicklers Children Admitted to Omdurman Pediatric Hospital

Abstract

This analytical, descriptive and cross-sectional study aim to evaluate the hematological parameters of sickle cell disease and sickle cell trait gene patients compared to control individuals admitted to Omdurman Pediatric hospital of Emergency in Khartoum state in the period between April 2011 to July 2011. One hundred (100) patients, and(50)controls were informed their parents about consent for participation was obtained. A venous blood of 2.5ml was collected in ethylene-diamine-tetra-acetic acid (EDTA) containers and investigated for sickle cell disease and sickle cell trait, a complete blood count (CBC) [semi automated hematological analyzer (Sysmex kx21N)]was used, also blood film, correction of WBCs, reticulocyte count, and hemoglobin electrophoresis were carried out, and statistical package for social sciences (SPSS) computer program version 16 was used for data processing. The percentage of HbAA according to the gender (70%) in the males and (30%) in the females, HbAS ( 20%) of the males (16%) of the females. HbSS (33%) and(28%) in the males and females respectively. HbSF (2%) of the males and (1%) of the females. The percentage of Hb type according to the age (30%) of HbAA were <5 years, (36%) were between 5 and 10 years and (34%) were >10 years. For HbAS (11%) were <5 years, (15%) were between 5 and 10 years and (10%) were > 10 years. 27% of HbSS were <5 years, 25% were between 5 and 10 years and 9% were >10 years. All the frequency of Hb SF (3%) were found to be <5 year. The means of Hb, RBCs & PCV were significantly increase in Hb AA than in Hb SS while the means of platelets count, reticulocytes count, & total leukocyte counts were significantly decrease in Hb AA than in Hb ASS. There were found significant increase in Hb AS than Hb AA in the neutrophil count with the p value (< 0.05) and mixed with the p value(<0.05). Other parameters show no difference between Hb AS and Hb AA. This results showed that means of Hb, RBCs & PCV were significantly decrease in Hb SF than in Hb AA while the means of PLTs count, absolute neutrophil and mixed were significantly increase in Hb SF than in Hb AA. The means of Hb, RBCs & PCV were significantly decrease in Hb SS than in Hb AS while the means of platelets count, reticulocytes count, & total leukocyte count were significantly increase in Hb SS than in Hb AS.