A study of Phytochemical and Antimicrobial Activity of Citrus paradisi and Citrus sinensis Seeds

Abstract

No wonder that grape fruit and orange are more popular fruits in Sudan. They are well known for their nutritional and medicinal properties, but for seeds oil of two plants fruit there is no enough studies reflecting the properties and benefits of them. This research aims to characterized and identify the properties and components of grape fruit and orange seeds oil. As the chemical analysis is important in formulating and developing new products and identifying the source of problems with unacceptable product in this work, phytochemical analysis, anti microbial activity, and physical properties were studied to identify the grape fruit and orange seeds oil. The Sudanese grape fruit (citrus pardisi) and orange (citrus sinensis) were collected from Algabraab field in November, 2014 the seeds were separated manually, dried at sunlight and grind. The oil was extracted by solvent extraction method using petroleum ether, a pale yellow colored oil (36.248)% for grape and(27.71)% for orange was obtained and characterized by physical properties and GC-Mass analysis . The crude extractions were obtained by solvent extraction method used methanol the yields percentage were (8.65) %for grape and (7.81)%for orange. The phytochemical screening was carried out using the methods described by Martinez & Valencia, (1999), Sofowora, (1993), Harborne, (1984) and Wall et.al, (1952)with some modifications. The TLC analysis of crude extract proved the presence of many chemical compounds. Antimicrobial activity which determined by disc diffusion method carried for all extractions showed good results. Fatty acids component like palmatic acid, linoleic and oleic and other compounds were found in the two oil samples. The physical properties results gave the grape fruit and orange seeds oil the vegetable oil behavior (can be used as edible oil), economic value health benefits and cosmetics manufacturing uses. The presences of saponin, alkaloids, tannins, favonoides, sterols, triterpens and cumarins in showed by phytochemical screening consider that the two planst seeds are active against bacteria and fungi